Abstract: The stability of the integrated profile of PSR B0329+54 at 1540MHz is studied with the observation data of Nanshan 25-m radio telescope up to 453 hours from 2003 to 2009. It is found that the profiles of two modes tend to stabilize at a rapid rate first, next change to a slower rate when the integration time reaches about (4±1)min, and then become rapidly stabilized again when the integration time reaches (140±60)min (normal mode) and (65±15)min (abnormal mode). The results of cross correlation coefficient show that the profile fluctuation of the two modes are not completely random. If transition time from a rapid to a lower stabilization rate is regarded as a criterion, the stability timescales of both two modes could be about 5min. The ratios between different pairs of components in the pulse profile fluctuate in different ways, suggesting that the three components in the profile follow different fluctuations. We discuss the possible reasons, such as observation noise and interstellar scintillation.